title for learn-about sections for chitons in A SNAIL'S ODYSSEY
  Adaptations to intertidal life
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  Gas exchange
  This hodge-podge selection of topics relating to intertidal life in chitons includes gas exchange, considered here, and TENACITY IN WAVES, TOLERANCE OF FRESHWATER, and LIGHT, DESICCATION, & TEMPERATURE STRESS, Considered in other sections.
 
Research study 1
 

photograph of lined chiton Tonicella lineataGas exchange in chitons occurs in ctenidia that hang freely into the pallial groove on either side of the body. Although it is something that might be expected, gill numbers may not be identical on either side of a chiton, at least not according to counts done on museum-preserved specimens of Tonicella lineata in California.  These show that individuals of 2-4cm in length have 24-32 ctenidia in the right pallial groove and 26-29 in the left pallial groove.  The means are about the same at 28, but whether there is any biological significance in the variability is not known.  Johnson 1969 Veliger 11: 272. Photo of Tonicella courtesy Ron Long, Simon Fraser University, Burnaby, British Columbia.

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Research study 2
  photograph of ctenidia of a gumboot chiton Cryptochiton stelleri drawing showing water flow through the pallial groove of a gumboot chiton Cryptochiton stelleriInvestigations at Friday Harbor Laboratories, Washington show that adult gumboot chitons Cyptochiton stelleri have about 75 ctenidia per side, while juveniles have about 50 per side.  Each ctenidium has about 100 filaments, providing a relative surface area for gas exchange that is notably large for a mollusc. Water is moved through the pallial cavity by ciliary action and flows from front to back.  In addition to irrigating the ctenidia, the flow as it moves along picks up urinary discharge from the kidney openings, reproductive products from the gonopores and, near the end of its route, feces from the anus.  

drawing to show counter-current flow arrangement in the ctenidium of a chiton Cryptochiton stelleriGases move across the ctenidial epithelium by diffusion in response to concentration gradients.  The authors demonstrate the existence of a counter-current exchange system in the ctenidial filaments.  Thus, hemolymph moves in one direction through the filaments, while seawater moves in the opposite direction. This means that oxygen-poor hemolymph entering the ctenidia first meets oxygen-depleted seawater exiting the filaments.  By the time the hemolymph leaves the filament it is richer in oxygen, but is now meeting seawater with maximum content of oxygen. The counter-current system ensures maximum concentration gradients between the two fluids along the width of each filament.  The gradients for exchange of carbon dioxide operate in reverse, but are likewise maximal. When exposed to moist air when the tide goes out, Cryptochiton may expose its ctenidia, apparently to facilitate aerial gas exchange.  However, measurements of oxygen uptake show rates in air only about one-quarter that in water, even at experimental temperatures in air (17-20oC) almost twice that in water (10oC).  Even when immersed, Cryptochiton often raises its girdle to maximise exchange of seawater within the pallial cavity.  Petersen & Johansen 1973 J Exp Mar Biol Ecol 12: 27.

NOTE the authors term this “blood”, but blood is defined as a tissue that circulates within closed systems of vessels and that usually contains cellular material involved in gas transport and internal defense.  Hemolymph is found in molluscs and arthropods, circulates in large, open (i.e., not enclosed in vessels) hemocoelic spaces, and carries few, if any, cellular elements.  A gas-transporting pigment hemocyanin is present in chitons, but is in solution and not contained within cells; see also Heath 1905 Biol Bull 9 (4): 213 for a detailed account of the excretory and circulatory systems in C. stelleri

 
Research study 3
 

photograph of chiton Nuttallina californica courtesy Ron Wolf, CaliforniaWhat modifications in morphology and functional mechanics of the ctenidia and pallial cavity are associated with increasing degree of air exposure in chitons?  If there is a parallel with what is seen in branchial-cavity morphology and functioning in crabs with increasing terrestriality, then we would expect adaptations along the following lines:

1) fewer ctenidia relative to body size
2) smaller volume of individual ctenidia
3) greater structural support for the ctenidia
4) vascularisation of the pallial groove
5) larger volume of the pallial groove.

photograph of lined chiton Tonicella lineataSo, what do we find in chitons?  A comparison of ctenidial and pallial-groove morphology in 2 species of chitons at Hopkins Marine Station, Pacific Grove, California, one of which, Nuttallina californica, inhabits the mid- to high-intertidal region, while the other, Tonicella lineata, lives mostly in the subtidal region, reveals the following:

1) Nuttallina of about 5cm length has 30-48 ctenidia on either side versus 22-29 in Tonicella of about 2-4cm length 
2) Nuttallina has more filaments per ctenidia than Tonicella, which, although not mentioned in the study, may represent a greater relative surface area for gas exchange in Nuttallina
3) Nuttallina has somewhat stouter ctenidia than Tonicella, possibly providing more support for the ctenidia in air
4) there is no evidence of vascularisation of the pallial cavity in either species
5) Nuttallina is capable of considerably more expansion of the pallial cavity than Tonicella, and does so during times of air exposure as long as the air is moist

So, 3 characteristics of Nuttallina identified in blue numbers in the above list support the notion of ctenidia/pallial cavity adaptations for more terrestrial life in chitons, while the other 2 don't. Is this enough, however, to suggest that the gas-exchanging capability in air of the high level-inhabiting Nuttallina is potentially greater than that of the low intertidal/subtidal-inhabitiing Tonicella?drawings showing different girdle/mantle orientations in chitons living subtidally (Tonicella lineata) and intertidally (Nuttallina californica)

Nuttallina’s girdle is additionally thick and muscular, and can be closed tightly over the ctenidia or raised up high, depending upon humity conditions (see drawings on Right).  This behaviour is also suggestive of an aerial oxygen-exchanging capacity.  Measurements of oxygen consumption in air in the 2 species show non-significant differences between the 2 species. Both species consume significantly less oxygen in air than in water.  The study is an interesting one and should repay further investigation.  Robbins 1975 Veliger 18(Suppl): 98.

 

photograph of a "duppy crab" Cardisoma guanhumi from BarbadosNOTE  in crabs the functionally analagous condition is known as “ballooning”, where the branchial chamber in terrestrial forms has become greatly expanded in size.  All of the adaptations listed for crabs increase the surface area available for gas-exchange in air



Caribbean "duppy crabs" Cardisoma guanhumi show "ballooning"
of the carapace surrounding the branchial chambers,
indicative of an ability for aerial oxygen uptake 0.3X

 
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